- $B%k!<%A%sB,Dj$O(B5mm$B&U(Bx$BD9$5(B18$B-QDxEY$N(BNMR$B;nNA4I$rMQ$$$^$9!#(B
- $BI,MW$K1~$8$F(B$BHyNL;nNA4I$GD4@=(B$B$7$^$9!#;nNAG;EY$O%W%m%H%sDL>oB,Dj$N>l9g!"J,;RNL$K$b$h$j$^$9$,(B2-3mg/0.5ml$B$"$l$P==J,$G$9!#$3$l$KBP$7$F%+!<%\%s$dFsl9g!"G;$$L\$K:n$C$F$*$-$^$9!#$?$@$7G;$9$.$k(B(100mg$B0J>e(B)$BMO1U$G$OJ,2rG=$,Dc2<$7$F$7$^$$$^$9!#(B
- $B;nNA4I$O=}$dOD$_$N$J$$$b$N$r!#(B
- $B%A%e!<%V$N8}$,$+$1$F$$$k$H!"MOG^$,4xH/$7$d$9$$$@$1$G$J$/!"2?$+$N>W7b$G$=$3$+$i3d$l$F$7$^$&2DG=@-$b$"$j!"BgJQ4m81$G$9!#(B
- $B;nNA$O??6u%]%s%W$GNI$/4%Ag$5$;$F$+$iD4@0$7$^$9!#(B
- $B;nNA$KMOG^$,;D$C$F$$$k$H$=$N%7%0%J%k$,%9%Z%/%H%k>e$K8=$l!";~$H$7$FL\E*$N%7%0%J%k$N4QB,$rK8$2$k$3$H$K$J$j$^$9!#(B1H-NMR$B$N?.9f6/EY(B($BLL@Q(B)$B$O%b%k?t$KHfNc$9$k$N$G!";nNA$h$j;DN1MOG^$,B?$1$l$P8e$BMOG^$O(BNMR$B@lMQ$N=E?eAG2=MOG^$r;H$$$^$9!#(B
- NMR$BAuCV$O!"B,Dj;~4VCf$N<'>l$r0lDj$KJ]$D$?$a!"MOG^$N=E?eAG$N(BNMR$B?.9f$r4F;k$7$F<'>l$rJd@5(B($B%m%C%/(B)$B$7$F$*$j!"$^$?!"B,Dj3+;OA0$K$O$=$N?.9f6/EY$rMQ$$$F@E<'>l(B(NMR$B$N<'@P(B)$B$rHy>/$J<'>l$GJd@5(B($B%7%`D4@0(B)$B$7$^$9!%(B
- $B%1%_%+%k%7%U%HI8=`J*$B4p=`J*$B;nNA$O6Q0l$KMO2r$5$;$^$9!#(B
- $BJ,2rG=$N9b$$NIl$rl9g$bJ,2rG=$,Dc2<$7$^$9!#MO1U$O%\%k%F%C%/%9%_%/%9%A%c!<$rMQ$$$F$8$e$&$V$s3Ie+$7$F$*$-$^$9!#(B
- $B;nNAMO1UNL$O(B5mm$B&U$G$ODl$+$i(B4.0$B!A(B5.0cm $B$KD4@0$7$^$9!#(B
- $BMOG^$,B?$9$.$k$HG;EY$,Dc$/$J$j!"B,Dj;~4V$,B?$/I,MW$K$J$C$F$7$^$$$^$9!#MOG^$,>/$J$9$.$k$H;nNA$N4QB,%3%$%k$KF~$kNN0h$r6Q0l$KJ]$F$:!"J,2rG=$,Dc2<$7$^$9!#(B
- $B%A%e!<%V@lMQ%-%c%C%W$r$7$C$+$j$HJD$a!"%Q%i%U%#%k%`$G%7!<%k$7$F$*$-$^$9!#(B
- $B=E%/%m%m%[%k%`MO1U$N>l9g$O;nNA4I%-%c%C%W$O%F%U%m%s@=$r$*4+$a$7$^$9!#(B
$B;nNA4I(B
- $B;nNA4I$K?^$N$h$&$J4z$r$D$1$F;nNAN,L>!"Fb@~!"L>A0$r5-F~$7$^$9!#(B
- $B;fJR$K;nNAL>$HFb@~!"L>A0$r=q$/(B
- $B%;%m%F!<%W!"%a%s%G%#%s%0%F!<%W$r;fJR$N>e$+$iE=$k!#%F!<%W$O:8$K(B1cm$B$O$_$@$5$;$F$*$/(B
- $B%A%e!<%V$KE=$k(B
- $B"((B $BG4CeLL$I$&$7$rE=$j9g$o$;$J$$$G$/$@$5$$!#(B
- $BG4CeLL$,$_$($F$$$k$HB>$N%5%s%W%k$KE=$jIU$-Mn2<$5$;$F3d$k$J$I$N;v8N$K$D$J$,$j$^$9$N$GG4CeLL$r;D$5$J$$$G2<$5$$!#(B
- $B$3$N4z$OB,Dj;~$K$O$:$7$^$9!#G4CeNO$,6/$9$.$k$H(B($B%S%K!<%k%F!<%W!"%,%`%F!<%W(B)$B$O$,$9$H$-$K%A%e!<%V$rGKB;$9$k4m81$,$"$j$^$9!#(B
- $BG4CeNO$N
$B?=9~%U%)!<%`(B
- $B%U%)!<%`$O(Belms$B$K%m%0%$%s$7$?>uBV$G%"%/%;%9$7$F$/$@$5$$!#(B
- $B?dDj9=B$<0$N2hA|%U%!%$%k$r(B$B:n@.0FFb(B$B$K=>$C$F$4MQ0U$/$@$5$$!#J]8n4p$J$I$NN,9f$O;H$o$:9=B$$GIA$$$F$/$@$5$$!#(B
- $B;nNAL>$O!"1Q?t;z$G(B10$BJ8;z0JFb$NN,>N$rM?$($F$/$@$5$$!#(B
- $B;nNANL$OMO1U$NG;EY$G$O$J$/!";nNA4I$NCf$KF~$C$F$$$k=E$5$G5-$7$F$/$@$5$$!%(B
- $BJ,;RNL$O!"J,;RNL$K0MB8$7$?B,Dj%Q%i%a!<%?$,$"$k$?$a$*?R$M$7$F$$$^$9!%(B
- 1.7mm$B&U;nNA4I$O%-%c%C%WItJ,$K%7%j%"%k%J%s%P!<$,?6$i$l$F$$$^$9!#$=$NHV9f$r%U%)!<%`$NHw9M$i$s$K5-F~$7$F$/$@$5$$!%(B
- $B;nNA$OB,Dj<e$N%9%?%s%I$KN)$F$F$*$$$F$/$@$5$$!%(B
$BB,Dj$,=*$o$j$^$7$?$i!"%U%)!<%`Aw?.85$N%a!<%k%"%I%l%9$K(BPDF$B$r$*Aw$j$9$k$+!"$^$?$O$*CN$i$;$7$^$9!#%5%s%W%k$H$"$l$P(BCD$B$r$*0z$-
$BEj9FMQ$J$I$K<+M3$K3HBg$r=q$-$?$$>l9g$J$I$O!"B,Dj@8%G!<%?$r$*EO$7$G$-$^$9$N$G!"?=9~MQ;f$K$=$N;]$*=q$-$/$@$5$$!#(B
$BCfB<@h@8$N%=%U%H(B$B$G$OFs$B%V%k%+!<$N%5%$%H(B$B$+$i!"B,Dj$HF1$8%=%U%H%&%(%"(BTopSpin$B$N%G!<%?=hM}$KFC2=$7$?%P!<%8%g%s$,%"%+%G%_%C%/%U%j!<$G;H$($^$9!%(BWindows$B!$(BMac$B!$(BLinux$BHG$,$"$j$^$9!%%U!<%j%(JQ49$7$?7k2L$r$=$N$^$^3+$/$3$H$,$G$-$k$[$+!$(BNMR$BB,Dj$N:]$KFI$_9~$^$l$?%G!<%?=hM}%Q%i%a!<%?$r$b$H$K%G!<%?$N:F=hM}$,MF0W$K9T$($^$9!%$^$?!$(BJEOL 270MHz$B$J$I$[$+$N5!$BB,Dj%G!<%?$N9=@.$O0J2<$N$h$&$K$J$C$F$*$j!"=hM}%=%U%H$G%9%Z%/%H%k%U%!%$%k$r3+$1$P!"%&%$%s%I4X?t$r$+$1$F%U!<%j%(JQ49$7$F!"2=3X%7%U%HJd@5!"%Y!<%9%i%$%sJd@5$J$I$N9T$o$l$?%9%Z%/%H%k$r3+$/$3$H$,$G$-$^$9!#(BFID$B%G!<%?$r3+$1$P!"%&%$%s%I4X?t$r$+$1$k$H$3$m$+$i$N=hM}$r$d$jD>$9$3$H$,$G$-$^$9!#(B
$B$?$H$($PB,Dj%U%!%$%kL>(B an3089$B!"l9g!"$*EO$7$7$?%9%Z%/%H%k$N1&B&$N%Q%i%a!<%?$NMs$N>e$N$[$&$K(B
- NAME an3089
- EXPNO 4
- PROCNO 1
$B$H=q$$$F$"$j$^$9!#(B
- /an3089/4/$B$N%U%)%k%@$r3+$/$H!"0lfid$B!!!!!!$H$$$&%U%!%$%k!!(B($B"($3$l$,(BFID$B%G!<%?$G$9!K(B
- acqu$B!!!!!!$H$$$&%U%!%$%k(B
- acqus$B!!!!!!$H$$$&%U%!%$%k(B
- pdata$B!!!!!!$H$$$&%U%)%k%@(B
- pdata$B%U%)%k%@$NCf$K(BPROCNO$B$N?t;z$NL>A0$N%U%)%k%@!"$3$3$G$O(B1$B$,$"$j!"$=$NCf$K0J2<$N%U%!%$%k$,$"$j$^$9!#(B
- 1r$B!!!!!!"($3$l$,%9%Z%/%H%k%G!<%?$G$9!#(B
- title$B!!!!%9%Z%/%H%k$N%?%$%H%k$G$9!#%F%-%9%H%(%G%#%?(B($B%a%bD"$J$I(B)$B$G3+$1$^$9!#(B
- $B$[$+$K%Q%i%a!<%?%U%!%$%k$,$"$j$^$9$,JQ99$r2C$($J$$$G$/$@$5$$!#(B
$BFsl9g!"%9%Z%/%H%k%U%!%$%k$O!!(B/an3089/4/pdata/1/2rr$B!"(BFID$B%U%!%$%k$O!"(B/an3089/4/ser$B!!$G$9!#(B
$BFs$B8=:_9T$J$C$F$$$k0MMjB,Dj(B$B$O2<5-$N$H$*$j$G$9!#(B
- $B0J2<$NB,Dj$K$D$$$F!$<<29!$9b29!$Dc29$G$NB,Dj$,2DG=$G$9!%(B
- 1H, 13CC, 15N$B$N4^$^$l$kB,Dj$O(B5mm$B&U$H(B1.7mm$B&U$N%5%s%W%k4I$NN>J}$,;HMQ2DG=$G$9!%$=$NB>$NB?3K$N4^$^$l$kB,Dj$O(B5mm$B&U$N$_$G$9!%(B
|
$B%W%m%H%s(B | $BDL>oB,Dj!"=E?eE:2C!"%G%+%C%W%j%s%0(B |
$B%+!<%\%s(BCOM$B!"(BDEPT | DEPT135$B!k$G$O(BCH3$B!"(BCH$B$,@5$K!"(BCH2$B$,Ii$K!"(BDEPT90$B!k$G$O(BCH$B$N$_$,=P$k$N$G!"N>
$B$=$NB>$N3K(B | 2H, 31P, 17O, 19F$B$J$I!%$*Ld$$9g$o$;$/$@$5$$!%(B
| $B29EY2DJQB,Dj(B | $B9=B$!$J?9U$NJQ2=$dMOG^%T!<%/$r$:$i$7$?$$$H$-$J$I(B |
$BMOG^>C5n(B | $BMOG^%T!<%/$NA*BrE*>H
NOE$B:9%9%Z%/%H%k(B | $B3K%*!<%P!<%O%&%6!<8z2L$K$h$k6u4VE*$K6a$$%W%m%H%s$N8!=P(B |
SPT$B:9%9%Z%/%H%k(B | $B%+%C%W%j%s%0%Q%?!<%s$N@:::(B |
STD$B%9%Z%/%H%k(B | $BK0OB0\F0:9%9%Z%/%H%k(B |
HHCOSY$B!"(BTOCSY | 1H-1H$B%7%U%HAj4XFs
| NOESY$B!"(BROESY | NOE$BAj4XFs
| J-$BJ,2r(B | $B%W%m%H%s$N(BJ$BJ,2r%9%Z%/%H%k(B |
HSQC | 1H-13C$B%7%U%HAj4XFs7k$7$?%W%m%H%s$H%+!<%\%s$r8!=P!%8!=P;~$K%G%+%C%W%j%s%0$r$;$:$K!$7k9gDj?t(B1JCH$B$rB,Dj$9$k$3$H$b2DG=$G$9(B
| HSQC-TOCSY | HSQC$BAj4X$K2C$(!$$=$N%W%m%H%s$HF1$8%9%T%s7O$KB0$7$F$$$k%W%m%H%s$r8!=P!%(BHSQC$B%T!<%/$N0LAj$rH?E>$5$;$?$j(B(IDR)$B
HSQC-NOESY$B!"(BHSQC-ROESY | HSQC$BAj4X$K2C$(!$$=$N%W%m%H%s$H6u4VE*$K6a$$%W%m%H%s$r8!=P!%DL>o$N(BNOESY$B!$(BROESY$B$G$O8!=P$7$K$/$$!$2=3X%7%U%H$,6a@\$7$F$$$k%W%m%H%sF1;N$N(BNOE$B!"(BROE$B8!=P$,!$(BHSQC$B%T!<%/$N%G%+%C%W%j%s%0$r$;$:$KB,Dj$9$k$3$H$G2DG=$K$J$j$^$9!%(B |
HMBC | $BFs!";07k9g$r2p$7$?%W%m%H%s$H%+!<%\%s$N%m%s%0%l%s%8Aj4X$r8!=P!%(B | $B=DJ}8~$NHO0O$r(B10$B!A(B20ppm$BDxEY$K8BDj$9$k%P%s%I%;%l%/%F%#%VB,Dj$b2DG=$G$9!#(B
| H2BC(HMQC-COSY) | 2$B7k9g$O$J$l$?(B1H-12C$B$^$?$O(BX-13C$B$r8!=P$7$^$9!%(BHMBC$B$G$OAj4X%T!<%/$rM?$($k(B1H-13C$B$,(B2$B7k9g$O$J$l$F$$$k$+(B3$B7k9gN%$l$F$k$+$ODL>o$O6hJL$,Fq$7$$$G$9$,!$(BH2BC$B$G$O!$$=$N%+!<%\%s$KD>7k$7$?%W%m%H%s$H!$(BJHH$B%+%C%W%j%s%0$N$"$k%W%m%H%s$KAj4X%T!<%/$r$"$?$($^$9!%(B
| 15N$B$H%W%m%H%s$N(BHMBC$B!$(BHSQC | $BI,MW$J@Q;;2s?t$O%+!<%\%s$N(B10$BG\DxEY$G$9!#(B
| J-$BJ,2r(BHMBC$B!"(BHETLOC | $B1s3V(BJCH$B7k9gDj?t$NB,Dj!%(B
| HRHMBC | $B1s3V(BJCH$B7k9g!$1s3V(BJHH$B7k9g$rFI$_ADEQUATE, INADEQUATE | 13C-13C$BAj4X%9%Z%/%H%k!#%+!<%\%s$N7k9g%M%C%H%o!<%/$N8!=P(B | |
T1, T2, T1$B&QB,Dj(B | $B4KOB;~4V$NB,Dj(B |
DOSY | $B=D<4$,3H;678?t$G$"$kFs
| | | |
($B"*(B$BB,DjJ}K!$NN,8l2r@b(B)
$B$3$NI=0J30$N$=$NB>$NB,Dj$K$D$$$F$b$4AjCL$/$@$5$$!%(B
$B$*4j$$(B
$BG@3XIt(BGC-MS$B!&(BNMR$BB,Dj<<(B(GC-MS & NMR Laboratory, Faculty of Agriculture)$B$GB,Dj$7$?%G!<%?$r0u:~8xI=$5$l$k>l9g$O!"$5$7$D$+$($J$$8B$j(B$BEvB,Dj<<$GB,Dj$7$?;]$rIU5-(B$B$7!"$=$NJL:~$j$r0lItB,Dj<<$K8f4sB#$/$@$5$k$h$&$*4j$$$7$^$9!#(B
$B$4$J$I$,$"$j$^$7$?$i(BGC-MS$B!&(BNMR$BB,Dj<<(B($BFb@~(B4134)$B$^$G$*4s$;$/$@$5$$!#(B