遺伝繁殖学研究室

　　　　　　　　　　　　　　　論文 　　　　　　　　　　　　　学会・講演＞

2022

1. Balboula A.Z., Aboelenain M., Sakatani M., Yamanaka K.I., Bai H., Shirozu T., Kawahara M., Hegab A.E.O., Zaabel S.M., and Takahashi M.  Effect of E-64 Supplementation during In Vitro Maturation on the Developmental Competence of Bovine OPU-Derived Oocytes.  Genes, 13: 1-10, doi: 10.3390/genes13020324, 2022.

2. Isoflavones and their metabolites influence the milk production ability of bovine mammary epithelial cells in a type-specific manner.  Tsugami Y., Wakasa H., Kawahara M., Nishimura T., and Kobayashi K.  Animal Science Journal, 93: e13720, 2022.

3. Cattle production by intracytoplasmic sperm injection into oocytes vitrified after ovum pick-up.  Kagawa S., Hiraizumi S., Bai H., Takahashi M., and Kawahara M.  Theriogenology, 185: 121-126, 2022.

4. Environmental factors affecting the conception rates of nulliparous and primiparous dairy cattle.  Ukita H., Yamazaki T., Yamaguchi S., Abe H., Baba T., Takahashi M., and Kawahara M., 105: 6947-6955, Journal of Dairy Science, 2022.

5. Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture.  Saito S., Akizawa H., Furukawa E., Yanagawa Y., Bai H., Takahashi M., and Kawahara M.  Journal of Reproduction and Development, 68: 330-334 2022. Cover Page.

6. Zona pellucida removal by acid Tyrode's solution affects pre- and post-implantation development and gene expression in mouse embryos.  Fan W., Huang T., Wu T., Bai H., Kawahara M., and Takahashi M.  Biology of Reproduction, 107: 1228-1241, 2022.

7. Recent progress of interferon-tau research and potential direction beyond
pregnancy recognition.  Bai H., Kawahara M., Takahashi M., and Imakawa K.
Journal of Reproduction and Development, 68: 299-306, 2022. REVIEW

2021

1.Tsugami Y., Wakasa H, Kawahara M., Watanabe A., Suzuki T., Nishimura T., and Kobayashi K. Adverse effects of LPS on membrane proteins in lactating bovine mammary epithelial cells. Cell and Tissue Research, 384: 435-448, 2021.

2.Tsugami Y., Wakasa H, Kawahara M., Nishimura T., and Kobayashi K. Lipopolysaccharide and lipoteichoic acid influence milk production ability via different early responses in bovine mammary epithelial cells. Experimental Cell Research, 400: 112472, 2021.

3.Saito S., Yamamura S., Kohri N., Bai H., Takahashi M., and Kawahara M.* Requirement for expression of WW domain containing transcription regulator 1 in bovine trophectoderm development. Biochemical and Biophysical Research Communications, 555: 140-146, 2021.

4.Kunii H., Kubo T., Asaoka N., Balboula A.Z., Hamaguchi Y., Shimasaki T., Bai H., Kawahara M., Kobayashi H., Ogawa H., and Takahashi M. Loop-mediated isothermal amplification (LAMP) and machine learning application for early pregnancy detection using bovine vaginal mucosal membrane. Biochemical and Biophysical Research Communications, 569: 179-186, 2021.

5.Murata H., Kunii H., Kusama K., Sakurai T., Bai H., Kawahara M., and Takahashi T. Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells. Biology of Reproduction, ioab143, 1-12, 2021.

6.Ho K.T., Homma K., Takanari J., Bai H., Kawahara M., Nguyen K.T.K., and Takahashi M. A standardized extract of Asparagus officinalis stem improves HSP70-mediated redox balance and cell functions in bovine cumulus-granulosa cells. Scientific Reports, 11: 18175, 1-17, 2021.

7.Fan W., Homma M., Xu R., Kunii H., Bai H., Kawahara M., Kawaguchi T., and Takahashi M. The use of a two-step removal protocol and optimized culture conditions improve development and quality of zona free mouse embryos. Biochemical and Biophysical Research Communications, 577: 116-123, 2021.

8.Hayashi Y., Saito S., Bai H., Takahashi M., and Kawahara M.* Mitochondrial maturation in the trophectoderm and inner cell mass regions of bovine blastocysts. Theriogenology, 175: 69-76, 2021.

9.Akizawa H., Saito S., Kohri N., Furukawa E., Hayashi Y., Bai H., Nagano M., Yanagawa Y., Tsukahara H., Takahashi M., Kagawa S., Kawahara-Miki R., Kobayashi H., Kono T., and Kawahara M.* Deciphering two rounds of cell lineage segregations during bovine preimplantation development. The FASEB Journal, 10: e21904, 2021.

10.
Komatsu M., Tsukahara H., Bai H., Takahashi M., Wakai T., and Kawahara M.* Cell-cycle dependent GATA2 subcellular localization in mouse 2-cell embryos. Biochemical and Biophysical Research Communications, 584: 1-6, 2021.


2020

1.Li J., Maeji M., Balboula A.Z., Aboelenain M., Fujii T., Moriyasu S., Bai H., Kawahara M., and Takahashi M.  Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos.  Journal of Reproduction and Development, 66: 9-17, 2020.

2.Li J., Balboula A.Z., Aboelenain M., Fujii T., Moriyasu S., Bai H., Kawahara M., and Takahashi M.  Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes.  Journal of Reproduction and Development, 66: 83-91, 2020.

3.Rahman A.M.I., Yamashita S., Islam M.R., Fujihara T., Yamaguchi H., Kawahara M., Takahashi M., Takahashi H., Gotoh T., and Yamauchi  N.  Type-I interferon regulates Matrix Metalloproteinases clearance of the bovine endometrial spheroid.  Animal Science Journal, 91: e13350, 2020.

4.Tsugami Y., Suzuki N., Kawahara M., Suzuki T., Nishimura T., and Kobayashi K.  Establishment of an in vitro culture model to study milk production and the blood-milk barrier with bovine mammary epithelial cells.  Animal Science Journal, 91: e13355, 2020.

5.Li G, Li J, Otsuka Y, Zhang S, Takahashi M, Yamada K.  A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets.  Materials (Basel), 13:677, 2020.

6.Balboula A.Z., Aboelenain M., Li J., Bai H., Kawahara M., Abdel-Ghani M.A., and Takahashi M.  Inverse relationship between autophagy and CTSK is related to bovine embryo quality.  Reproduction, 159: 757-766, 2020.

7.Bai H., Hiura H., Obara Y., Kawahara M., and Takahashi M.  Menaquinone-4 (vitamin K2) induces proliferation responses in bovine peripheral blood mononuclear cells.  Journal of Dairy Science, 103: 7531-7534, 2020.

8.Kohri N.,  Akizawa H., Iisaka S., Bai H., Takahashi T., and Kawahara M.  The role of RHOA signaling in trophectoderm cell-fate decision in cattle.  Biochemical and Biophysical Research Communications, 528: 713-718, 2020.

9.Balboula A.Z., Schindler K., Kotani T., Kawahara M., and Takahashi M.  Vitrification-induced Activation of Lysosomal Cathepsin B Perturbs Spindle Assembly Checkpoint Function in Mouse Oocytes.  Molecular Human Reproduction, 26: 689-701, 2020.

10.Yamamura S., Goda N., Akizawa H., Kohri N., Balboula A.Z., Kobayashi K., Bai H., Takahashi M., and Kawahara M.  Yes-associated protein 1 translocation through actin cytoskeleton organization in trophectoderm cells.  Developmental Biology, 468: 14-25, 2020.
プレスリリース: https://www.hokudai.ac.jp/news/pdf/200930_pr2.pdf

11.Bai H., Ukita H., Kawahara M., Mitani T, Furukawa E., Yanagawa Y., Yabuuchi N, Kim H, Takahashi M.  Effect of summer heat stress on gene expression in bovine uterine endometrial tissues.  Animal Science Journal, 2020, IN PRESS. 




2019

1.Akizawa H., Yanagawa Y., Nagano M., Bai H., Takahashi M. and Kawahara M.  Significance of CCN2 expression in bovine preimplantation development.  Animal Science Journal, 90: 49-54, 2019.

2.Shiina K., Komatsu M., Yokoi F., Bai H., Takahashi M. and Kawahara M.  Overgrowth of mice generated from postovulatory aged oocyte spindles. FASEB BioAdvances, 1: 393-403, 2019.

3.Bai H., Talukder M.A.S., Kunii H., Itoh T., Kawahara M. and Takahashi M.  Evaluation of the immune status of peripheral blood monocytes from dairy cows during the periparturition period.  Journal of Reproduction and Development, 65: 313-318, 2019.

4.Oikawa K., Yamazaki T., Yamaguchi S., Abe H., Bai H., Takahashi M., and Kawahara M.  Effects of use of conventional and sexed semen on the conception rate in heifers: a comparison study.  Theriogenology, 135: 33-37, 2019.

5.Kohri N., Akizawa H., Iisaka S., Bai H., Yanagawa Y., Takahashi M., Komatsu M., Kawai M., Nagano M., and Kawahara M.  Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst.  Journal of Biological Chemistry, 294: 19209-19223, 2019.　プレスリリース: https://www.hokudai.ac.jp/news/191121_pr.pdf




2018

1. Yamanaka KI, Khatun H, Egashira J, Balboula AZ, Tatemoto H, Sakatani M, Takenouchi N, Wada Y, Takahashi M. Heat-shock-induced cathepsin B activity during IVF and culture compromises the developmental competence of bovine embryos. Theriogenology, 114: 293-300, 2018.

2. Talukder MAS, Balboula AZ, Shirozu T, Kim SW, Kunii H, Suzuki T, Ito T, Kimura K, Takahashi M. Activation of lysosomal cathepsins in pregnant bovine leukocytes. Reproduction, 155: 515-528, 2018.

3. Kikuchi K., Sasaki K., Akizawa H., Tsukahara H., Bai H.,  Takahashi M., Nambo Y., Hata H. and Kawahara M.  Identification and expression analysis of cDNA encoding insulin like growth factor 2 in horse.  Journal of Reproduction and Development, 64: 57-64, 2018.

4. Kikuchi K., Kozai K., Hojo T., Sakatani M., Okuda K., Bai H., Kawahara M., and Takahashi M.  Evaluating the electrical impedance and mucus-related gene expression of uterine endometrial tissues in mares.  Journal of Reproduction and Development, 64: 193-197, 2018.

5. Akizawa H., Kobayashi K., Bai H., Takahashi M., Kagawa S., Nagatomo H. and Kawahara M.  Reciprocal regulation of TEAD4 and CCN2 for the trophectoderm development of the bovine blastocyst. Reproduction, 155: 563-571, 2018.

6. Nakahara K., Sakuma S., Kawahara M., Takahashi M., and Arai F.  Time-lapse mechanical characterization of zona pellucida using a cell carrier chip.  Journal of Microelectromechanical Systems, 27: 464-471, 2018.

7. Kunii H., Koyama K., Ito T., Suzuki T., Balboula A.Z., Shirozu T., Bai H., Nagano M., Kawahara M., and Takahashi M.  Pregnancy-induced expression of interferon-stimulated genes in cervical and vaginal mucosal membrane.  Journal of Dairy Science,101: 8396-8400, 2018.

8. Musavi S.A.A., Yamashita S., Fujihara T., Masaka H., Islam M.R., Kim S.,  Goto T., Kawahara M., Tashiro K., and Yamauchi N.  Analysis of differentially expressed genes and the promoters in bovine endometrium throughout estrus cycle and early pregnancy.  Animal Science Journal, 89: 1609-1621, 2018.

9. Suzuki T., Sakumoto R., Hayashi K.G., Ogiso T., Kunii H., Shirozu T., Kim S.W., Bai H., Kawahara M., Kimura K., and Takahashi M.  Involvement of IFNT in the induction of apoptotic, pyroptotic and autophagic cell death-related signaling pathways in bovine uterine endometrium during early pregnancy.  Journal of Reproduction and Development, 64: 495-502, 2018.



2017

1.Balboula AZ, Blengini CS, Gentilello AS, Takahashi M, Schindler K, Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion. Biol Reprod. 96: 1197-1209. 2017

2.Shirozu T., Iwano H., Ogiso T., Suzuki T., Balboula  AZ., Bai H., Kawahara M., Kimura K., Takahashi H., Bai R., Kim SW., Yanagawa Y., Nagano M., Imakawa K., Takahashi M.  Estrous cycle stage-dependent manner of type I interferon-stimulated genes 1 induction in the bovine endometrium. Journal of Reproduction and Development. 63: 211-220, 2017.

3.Aboelenain M., Balboula AZ., Kawahara M., Montasser Ael-M., Zaabel SM., Kim SW., Nagano M., Takahashi M．Pyridoxine supplementation during oocyte maturation improves the developmental rate and quality of bovine preimplantation embryos.　Theriogenology  91: 127-133, 2017

4.Sakatani M., Yamanaka K., Balboula AZ., Takahashi M.,  Different thermotolerance in in-vitro-produced embryos derived from different maternal and paternal genetic backgrounds. Anim Sci J.  doi: 10.1111/asj.12875.2017.

5.Kaneda M., Takahashi M., Yamanaka K., Saito K., Taniguchi M., Akagi S., Watanabe S., Nagai T. Epigenetic analysis of bovine parthenogenetic embryonic fibroblast cells. Journal of Reproduction and Development. 63:365-375, 2017.

6.Yan W., Kanno C., Oshima E., Kuzuma Y., Kim S.W., Bai H., Takahashi M., Yanagawa Y., Nagano M., Wakamatsu J.I., and Kwahara M.Enchancement of sperm motility and viability by turmeric by-product dietary suplimentation in roosters. Animal Reproduction Science, 185: 195-204, 2017.



2016

1.Shirozu T., Sasaki K., Kawahara M., Yanagawa Y., Nagano M., Yamauchi N., and Takahashi M. Expression dynamics of bovine MX gene in the endometrium and placenta during early to mid-pregnancy. Journal of Reproduction and Development, 62: 29-35, 2016.

2.Sasaki K., Tanaka A, Nagatomo H., Ogawa H., Kobayashi K., Takahashi M., and Kawahara M. Characterization of Toll-Like Receptor 9 expression during mouse preimplantation development. Journal of Genital System & Disorders, 5: 1-5, 2016.

3.Nagatomo H., Kohri N., Akizawa H., Hoshino Y., Yamauchi N., Kono T., Takahashi M., and Kawahara M. Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. Animal Science Journal, 87: 457-461, 2016.

4.Akizawa H., Nagatomo H., Odagiri H., Kohri N., Yamauchi N., Yanagawa Y., Nagano M., Takahashi M., and Kawahara M. Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts. Molecular Reproduction and Development, 83: 516-25, 2016.

5.Sakatani M., Takahashi M., Takenouchi N., The efficiency of vaginal temperature measurement to detect the estrus in Japanese Black cows. J Reprod Dev.62: 201-207, 2016.

6.Kozai K., Tokuyama S., Szóstek A.Z., Toishi Y., Tsunoda N., Taya K., Sakatani M., Takahashi M., Nambo Y., Skarzynski D., Yamamoto Y., Kimura K., and Okuda K. Evidence for a PGF2α auto-amplification system in the endometrium in the mare. Reproduction, 151: 517-526, 2016.

7.Yamazaki W., Amano T., Bai H., Takahashi M., and Kawahara M. The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice. The Journal of Biological Chemistry, 291: 20924-20931,, 2016.



2015

1.Sakatani M, Yamanaka K, Balboula AZ, Takenouchi N, Takahashi M. Heat stress during in vitro fertilization decreases fertilization success by disrupting anti-polyspermy systems of the oocytes. Molecular Reproduction and Development, 82 :36-47, 2015.

2.Aboelenain M., Kawahara M., Balboula A.Z., Montasser A.E., Zaabel S.M., Okuda K., and Takahashi M. Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. Journal of Reproduction and Development, 61: 229-236, 2015.

3.Nagatomo H., Akizawa H., Sada A., Kishi Y., Yamanaka K.I., Takuma T., Sasaki K., Yamauchi N., Yanagawa Y., Nagano M., Kono T., Takahashi M., and Kawahara M. Comparing spatial expression dynamics of bovine blastocyst under three different procedures: in-vivo, in-vitro, and somatic cell nuclear transfer embryos. The Japanese Journal of Veterinary Research, 63: 159-171, 2015..

4.Kawahara M., Koyama S., Iimura S., Yamazaki W., Tanaka A., Kohri N., Sasaki K., and Takahashi M. Preimplantation death of xenomitochondrial mouse embryo harbouring bovine mitochondria.
Scientific Reports, 5:14512.doi:10.1038/srep14512., 2015.
.

解説　夏季の暑熱と人工受精受胎率.　高橋昌志．LIAJ news, 153:6-8.

総説　夏季の暑熱ストレスによる繁殖性への影響．高橋昌志．臨床獣医、5月号.12-17．



2014

1. Bai H, Sakurai T, Godkin JD, Imakawa K. Expression and in situ localization of GATA4, 5 and 6 mRNAs in ovine conceptuses and uterine endometria during the peri-implantation period. Animal Sciences Journal, 85: 388-394, 2014.

2. Bai H, Sakurai T, Bai R, Yamakoshi S, Aoki E, Kuse M, Okuda K, Imakawa K. Establishment and characterization of immortalized bovine endometrial epithelial cells. Animal Sciences Journal, 85: 799-804, 2014.

3. Bai R, Bai H, Kuse M, Ideta A, Aoyagi Y, Fujiwara H, Okuda K, Imakawa K, akurai T. Involvement of VCAM-1 in the bovine conceptus adhesion to the uterine endometrium. Reproduction,148:119-127, 2014.

4. Tachibana Y, Sakurai T, Bai H, Shiota K, Nambo Y, Nagaoka K, Imakawa K. RNA-seq analysis of equine conceptus transcripts during embryo fixation and capsule disappearance. PLoS One, 9: e114414, 2014.

5.Yamazaki W, Takahashi M, Kawahara M. Restricted development of mouse triploid fetuses with disorganized expression of imprinted genes. Zygote, [Epub ahead of print], 2014

6.Yamagami K, Yamauchi N, Kubota K, Nishimura S, Chowdhury VS, Yamanaka K, Takahashi M, Tabata S, and Hattori MA. Expression and regulation of Foxa2 in the rat uterus during early pregnancy. J Reprod Dev, 60:468-475, 2014.

7.Kozai K, Hojo T, Tokuyama S, Szóstek AZ, Takahashi M, Sakatani M, Nambo Y, Skarzynski DJ, and Okuda K. Expression of aldo-keto reductase 1C23 in the equine corpus luteum in different luteal phases. J Reprod Dev, 60 :150-154, 2014.

8.Takahashi H, Tsunazaki M, Hamano T, Takahashi M, Okuda K, Inumaru S, Okano A, Geshi M, Hirako M. Biological activity of recombinant bovine interferon τ produced by a silkworm-baculovirus gene expression system. J Vet Med Sci, 76 :4474-4451, 2014.



2013

1.Nakagawa S, Bai H, Sakurai T, Nakaya Y, Konno T, Miyazawa T, Gojobori T,　Imakawa K. Dynamic evolution of endogenous retrovirus-derived genes expressed in bovine conceptuses during the period of placentation. Genome Biology and Evolution, 5: 296-306, 2013.

2.Sakurai T, Bai H, Bai R, Sato D, Arai M, Okuda K, Ideta A, Aoyagi Y, Godkin JD, Imakawa K. Down-regulation of interferon tau gene transcription with a transcription factor, EOMES. Molecular Reproduction and Development, 80: 371-383, 2013.

3.Kim MS, Sakurai T, Bai H, Bai R, Sato D, Nagaoka K, Chang KT, Godkin JD, Min KS, Imakawa K. Presence of transcription factor OCT4 limits interferon-tau expression during the pre-attachment period in sheep. Asian-Australasian Journal of Animal Sciences, 26: 638-645, 2013.

4.Sakurai T, Nakagawa S, Kim MS, Bai H, Bai R, Li J, Min KS, Ideta A, Aoyagi Y, Imakawa K. Transcriptional regulation of two conceptus interferon tau genes expressed in japanese black cattle during peri-implantation period. PLoS One, 8: e80427, 2013.

5.Aono A., Nagatomo H., Takuma T., Nonaka R., Ono Y., Wada Y., Abe Y., Takahashi M., Watanabe T. and Kawahara M. Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle. Theriogenology, 79: 1146-1152, 2013.

6.Sasaki K., Yoneda A., Ninomiya A., Kawahara M. and Watanabe T. Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631. Biochemical and Biophysical Research Communications, 430: 161-166, 2013.

7.Nagatomo H., Kagawa S., Kishi Y., Takuma T., Sada A., Yamanaka KI., Abe Y., Wada Y., Takahashi M., Kono T. and Kawahara M. Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle. Biology of Reproduction, DOI 10.1095/biolreprod.113.108993, 2013.

8.Sugawara S, Ito T, Sato T, Yokoo M, Mori Y. Kasuga K, Kojima I, Fukuda T, Yamanaka K, Sakatani M, Takahashi M, Kobayashi M. Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds. Animal Science Journal. 84:275-280. 2013.

9.Balboula AZ, Yamanaka K, Sakatani M, Kawahara M,Hegab AO, Zaabel SM, Takahashi M, Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus?oocyte complexes exposed to heat shock during in vitro maturation. Reproduction. 146:407-417, 2013.

10.Sasaki K., Kawahara M. and Watanabe T. Characteristics of mx gene that shows resistance to vesicular stomatitis virus from avians to mammals. Advances in Genetics Research, NOVA Science publishers, New York, USA, Chapter 6, 11:p117-130, 2013.

11.Sasaki K., Tungtrakoolsub P., Morozumi T., Uenishi H., Kawahara M. and Watanabe T. A single nucleotide polymorphism of porcine MX2 gene provides antiviral activity against vesicular stomatitis virus. Immunogenetics, 66 :25-32, 2014.

家畜の繁殖研究の流れと展望、高橋昌志、日本胚移植学雑誌、35巻１－８．



2012

1.Takahashi M, A review: Effect of intracellular redox status and its regulation on bovine embryonic development. Journal of Reproduction and Development.58:1-9.2012.

2.Sakatani M, Alvarez NV, Takahashi M, Hansen PJ. Consequences of physiological heat shock beginning at the zygote stage on embryonic development and expression of stress response genes in cattle. Journal of Dairy Science. 95:3080-91. 2012.

3.Matsunari H, Maehara M, Nakano K, Ikezawa Y, Hagiwara Y, Sasayama N, Shirasu A, Ohta H, Takahashi M, Nagashima H. Hollow fiber vitrification: a novel method for vitrifying multiple embryos in a single device. Journal of Reproduction and Development. 58:599-608, 2012.

4.Maehara M, Matsunari H, Honda K, Nakano K, Takeuchi Y, Kanai T, Matsuda T, Matsumura Y, Hagiwara Y, Sasayama N, Shirasu A, Takahashi M, Watanabe M, Umeyama K, Hanazono Y, Nagashima H. Hollow fiber vitrification provides a novel method for cryopreserving in vitro maturation/fertilization-derived porcine embryos. Biology of Reproduction. 87:133. doi: 10.1095/biolreprod.112.100339.2012.

5.Kozai K, Hojo T, Takahashi M, Acosta TJ, Nambo Y, Okuda K. Seasonal Changes in Luteal Progesterone Concentration and mRNA Expressions of Progesterone Synthesis-Related Proteins in the Corpus Luteum of Mares. Journal of Reproduction and Development. 58:393-397, 2012.

6.Kawahara M. and Kono T. Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny. Journal of Reproduction and Development, 58: 175-179, 2012.



2011

1.Yamanaka K, Kaneda M, Inaba, Y, Saito K, Bubota K, Sakatani M, Sugimura S, Imai, K,Watanabe S, Takahashi M. DNA methylation analysis on satellite I region in blastocysts obtained from somatic cell cloned cattle. Animal Science Journal , 82:523-530.2011.

2.Yamanaka K, Sakatani M, Kubota K, Balboula AZ, Sawai K, Takahashi M,　Effects of downregulating DNA methyltransferase 1 transcript by RNA interference on DNA methylation status on satellite I region and in vitro development of bovine somatic cell nuclear transfer embryos. Journal of Reproduction and Development. 57: 393-402.2011.

3.Nakano K, Matsunari H, Nakayama N, Ogawa B, Kurome M, Takahashi M, Matsumoto M, Murakami H, Kaji Y, Nagashima H, Cloned porcine embryos can maintain developmental ability after cryopreservation at the morula stage. Journal of Reproduction and Development. 57:312-316.2011.

4.Sawai K, Takahashi M, Fujii T, Moriyasu S, Hirayama H, Minamihashi A, Hashizume T, Onoe S. DNA methylation status of bovine blastocyst obtained from various procedures. Journal of Reproduction and Development, 57:236-241.2011.

5.Sakatani M, Balboula AZ, Yamanaka K, Takahashi M, Effect of summer heat environment on body temperature, estrous cycles and blood antioxidant levels in Japanese Black cow, Animal Science Journal, 82:523-530.2011.

6.Takahashi M, A review: Heat stress on reproductive function and fertility in mammals, Reproducive Medicine and Biology. DOI10. 1007/s12522-011-0105-6. 2011.



2010

1.Sawai K, Takahashi M, Moriyasu S, Hirayama H, Minamihashi A, Hashizume T, Sadao Onoe S, Changes in the DNA methylation status of bovine embryos from the blastocyst to elongated stage derived from somatic cell nuclear transfer. Cloning and Stem cells. 12:15-22. 2010.

2.Yamanaka K, Balboula AZ, Sakatani M, Takahashi M, Gene Silencing of DNA Methyltransferases by RNA Interference in Bovine Fibroblast Cells. Journal of Reproduction and Development. 56:60-67. 2010.

3.Balboula AZ, Yamanaka K, Sakatani M, Hegab AO, Zaabel SM, Takahashi M. Cathepsin B activity is related to the quality of bovine cumulus oocyte complexes and its inhibition can improve their developmental competence. Molecular Reproduction and Development. 77:439-448. 2010.

4.Tani M, Hayashida T, Tomokawa K, Mito Y, Funakoshi D, Tani C, Sakatani M, Takahashi M, Kitahara G, Kamimura S. Effect of embryo transfer following artificial insemination (ETFAI) on reproductive performance in dairy cows in South-Western Japan. Journal of Veterinary Medical Science. 72:627-629. 2010.

5.Kubota K, Yamauchi N, Yamagami K, Nishimura S, Gobaru T, Yamanaka K, Wood C, Soh T, Takahashi M, Hattori MA, Steroidal regulation of Ihh and Gli1 expression in the rat uterus, Cell and Tissue Research. 40:389-395. 2010.

6.Balboula AZ, Yamanaka K, Sakatani M, Hegab AO, Zaabel SM, Takahashi M. Compromising role of intracellular cathepsin B on the quality and developmental competence of bovine preimplantation embryos, Molecular Reproduction and Development.77:1031-1039. 2010.

7.Siemieniuch MJ, Majewska M, Takahashi M, Sakatani M, ?ukasik K, Okuda K, Skarzynski DJ, Are glucocorticoids auto- and/or paracrine factors in early bovine embryo development and implantation? Reproductive Biology 10:249-256. 2010.

8.Kawahara M., Wu Q. and Kono T. Involvement of insulin-like growth factor 2 in angiogenic factor transcription in bi-maternal mouse conceptuses. Journal of Reproduction and Development, 56: 79-85, 2010.

9.Sugimura S., Yamanaka K., Kawahara M., Wakai T., Yokoo M. and Sato E. Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos. Animal Science Journal, 81: 48-57, 2010.

10.Kawahara M. and Kono T. Longevity in mice without a father. Human Reproduction, 25: 457-461, 2010.

11.Sugimura S., Yokoo M., Yamanaka KI., Kawahara M., Moriyasu S., Wakai T., Nagai T., Abe H. and Sato E. Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos. Cellular Reprogramming, 12: 463-474, 2010.



2009

1.Sakurai T, Sakamoto A, Muroi Y, Bai H, Nagaoka K, Tamura K, Takahashi T, Hashizume K, Sakatani M, Takahashi M, Godkin JD, Imakawa K. Induction of Endogenous Tau Interferon Gene Transcription by CDX2 and High Acetylation in Bovine Non-Trophoblast Cells. Biology of Reproduction. 80:1223-1231, 2009.

2.Bai H, Sakurai T,Kim MS, Muroi Y,Ideta A, Aoyagi Y, Nakajima H, Takahashi M, Nagaoka K, Imakawa K, InvolvementQ1 of GATA Transcription Factors in the Regulation of Endogenous Bovine Interferon-Tau Gene Transcription, Molecular Reproduction and Development, 76:1143-1152, 2009.

3.Ogawa H., Shindo N., Kumagai T., Usami Y., Shikanai M., Jonwn K., Fukuda A., Kawahara M., Sotomaru Y., Tanaka S., Arima T. and Kono T. Developmental Ability of Trophoblast Stem Cells in Uniparental Mouse Embryos. Placenta, 30: 448-456, 2009.

4.Kawahara M., Morita S., Takahashi N. and Kono T. Defining contributions of paternally methylated imprinted genes at the IGF2-H19 and DLK1-GTl2 domains to mouse placentation by transcriptomic analysis. The Journal of Biological Chemistry, 284: 17751-17765, 2009.

5.Yamanaka K.I., Sugimura S., Wakai T., Kawahara M. and Sato E. Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos. Journal of Reproduction and Development, 55: 638-644, 2009.

βメルカプトエタノールが毒劇物指定を受けての家畜生殖細胞操作・培養への対応.　高橋昌志．日本胚移植学雑誌31:127-133．

体細胞クローン作出におけるエピジェネティックス研究の現状．山中賢一、高橋昌志、日本胚移植学雑誌30:99-105．



2008

1.Sakatani M, Yamanaka K, Kobayashi S, Takahashi M,. Heat shock-derived reactive oxygen species induce embryonic mortality in in vitro early stage bovine embryos. Journal of Reproduction and Development, 54,486-501, 2008.

2.Wakai T., Tanaka H., Yamanaka K.I., Sugimura S., Sasada H., Kawahara M., Kobayashi E., and Sato E. Induction of estrus in pubertal miniature gilts. Animal Reproduction Science, 103: 193-198, 2008.

3.Sugimura S., Kawahara M., Wakai T., Yamanaka K.I., Sasada H., and Sato E. Effect of cytochalasins B and D on the developmental competence of somatic cell nuclear transfer embryos in miniature pigs. Zygote, 16: 153-159, 2008. *These authors contributed equaly to this work.

4.Kawahara M., Obata Y., Sotomaru Y., Shimozawa N., Bao S., Tsukadaira T., Fukuda A. and Kono T. Protocol for the production of viable bi-maternal mouse embryos. Nature Protocols, 3: 197-209, 2008.

5.Jincho Y., Sotomaru Y., Kawahara M., Ogawa H., Obata Y., and Kono T. Identification of Genes Aberrantly Expressed in Mouse Embryonic Stem Cell-Cloned Blastocysts. Biology of Reproduction, 78: 568-576, 2008.

6.Wakai T., Sugimura S., Yamanaka K.I., Kawahara M., Sasada H., Tanaka H., Ando A., Kobayashi E., and Sato E. Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries. Cloning and Stem Cells, 10: 249-262, 2008.

7.Wu Q., Kawahara M. and Kono T. Synergistic role of Igf2 and Dlk1 in fetal liver development and hematopoiesis in bi-maternal mice. Journal of Reproduction and Development, 54: 177-182, 2008.



2007

1.Sakatani M, Suda I, Oki T, Kobayashi S, Kobayashi S, Takahashi M, Effects of purple sweet potato anthocyanins on development and intracellular redox status of bovine preimplantation embryos exposed to heat shock. Journal of Reproduction and Development. 53:605-614, 2007.

2.Suzuki C, Yoshioka K, Sakatan M, Takahashi M, Glutamine and Hypotaurine Improves Intracellular Oxidative Status and In Vitro Development of Porcine Preimplantation Embryos. Zygotes. 15:317-324. 2007.

3.Kobayashi S, Sakatani M, Kobayashi S, Okuda K, Takahashi M, Gene Silencing of Cyclooxygenase-2 mRNA by RNA Interference in Bovine Cumulus-Granulosa cells. Journal of Reproduction and Development. 53: 1305-1311, 2007.

4.Matsumoto S, Okumura K, Ogata A, Hisatomi Y, Sato A, Hattori K, Matsumoto M, Kaji Y, Takahashi M, Yamamoto T, Nakamura K, Endo F, Isolation of tissue progenitor cells from duct-ligated salivary glands of swine. Cloning and Stem Cells. 9:176-190, 2007.

5.Kawahara M., Wu Q., Takahashi N., Morita S., Yamada K., Ito M., Ferguson-Smith A.C., and Kono T. High-frequency generation of viable mice from engineered bi-maternal embryos. Nature Biotechnology, 25: 1045-1050, 2007.

6.Kawahara M., Wu Q., Ferguson-Smith A.C., and Kono T. Appropriate expression of paternally methylated imprinted genes on chromosome 12 leads to extended development of bi-maternal embryos to term but lethal. FEBS letters, 581: 5178-5184, 2007.

7.Wakai T., Kawahara M., Yamanaka K.I., Hoshino Y., Tomioka I, Sugimura S., Sugawara A., and Sato E. Morphological aspects of porcine embryos reconstructed by somatic cell nuclear transfer. Morphology, 2, 23-24, 2007.