Laboratory of Molecular Enzymology

Molecular-Enzymology

Molecular analysis of carbohydrate-active enzymes and synthesis of saccharides

Faculty

  • NameE-mail Address
  • Position
  • Degree
  • KIMURA Atsuo
  • Professor
  • Ph.D.

kimura[at]abs.agr.hokudai.ac.jp

Researchers’ directory
  • OKUYAMA Masayuki
  • Lecturer
  • Ph.D (Agriculture)

okuyama[at]abs.agr.hokudai.ac.jp

Researchers’ directory
  • TAGAMI Takayoshi
  • Assistant Professor
  • Ph.D (Agriculture)

tagami[at]abs.agr.hokudai.ac.jp

Researchers’ directory

Website

http://www.agr.hokudai.ac.jp/en/rfoa/abs/abs2-3.html

Selected Publications

  1. Ma M, Okuyama M, Tagami T, Kikuchi A, Klahan P, Kimura A: α-1,3/α-1,4-Glucosidase from Aspergillus niger Exhibits Unique Transglucosylation to Generate High Levels of Nigerose and Kojibiose. Journal of Agricultural and Food Chemistry, 67:3380-3388, 2019.
  2. Klahan P, Okuyama M, Jinnai K, Ma M, Kikuchi A, Kumagai Y, Tagami T, Kimura A: Engineered dextranase from Streptococcus mutans enhances the production of longer isomaltooligosaccharides. Bioscience, Biotechnology, and Biochemistry, 82:1480-1487, 2018.
  3. Kikuchi A, Okuyama M, Kato K, Osaki S, Ma M, Kumagai Y, Matsunaga K, Klahan P, Tagami T, Yao M, Kimura A: A novel glycoside hydrolase family 97 enzyme: Bifunctional β-l-arabinopyranosidase/α-galactosidase from Bacteroides thetaiotaomicron. Biochimie, 142:41-50, 2017.
  4. Okuyama M, Matsunaga K, Watanabe KI, Yamashita K, Tagami T, Kikuchi A, Ma M, Klahan P, Mori H, Yao M, Kimura A: Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b). FEBS Journal, 284:766-783, 2017.
  5. Tagami T, Miyano E, Sadahiro J, Okuyama M, Iwasaki T, Kimura A: Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl. The Journal of Biological Chemistry, 291:16438-16447, 2016

Specialized Fields

Research Keywords

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