Program and Schedule

Saturday, July 10, 2004
1500-1800		Registration & Poster Set-up (Conference Hall, Hokkaido University)
1800-2000		Welcome Reception & Registration (Sapporo Aspen Hotel)

Sunday, July 11, 2004
0700-0845		Breakfast (Sapporo Aspen Hotel)
0800-0845		Poster Set-up
0845-0915		Opening Remarks & Announcements Seizo Fujikawa (Hokkaido University, Sapporo, Japan) Matsuo Uemura (Iwate University, Morioka, Japan) Kiyoaki Maruyama (Director General of National Agricultural Research Center for Hokkaido Region, National Agriculture Research Organization, Sapporo, Japan)
0915-1015		Keynote Lecture (Chair, Matsuo Uemura) Kazuo Shinozaki (RIKEN, Tsukuba, Japan) Global analysis of gene networks to solve complex biotic stress responses
1015-1045		Coffee Break
1045-1215		Session #1: Signal Transduction & Gene Regulation (Chair, Charles Guy)
1045-1130		Glenn Thorlby (University of London, Egham, UK) The roles of the SFR2 and SFR3 proteins in freezing tolerance
1130-1215		Norio Murata (National Institute of Basic Biology, Okazaki, Japan) Cold-inducible gene expression and cold sensors in cyanobacteria
1215-1230		Group Photo
1230-1400		Lunch
1400-1730		Session #1: Signal Transduction & Gene Regulation (Chair, Norio Murata)
1400-1445		Kazuko Yamaguchi-Shinozaki (Japan International Research Center for Agricultural Sciences, Tsukuba, Japan) Regulation of gene expression and signaling pathway under cold and drought stress condition
1445-1530		Mike Hasegawa (Purdue University, West Lafayette, USA) Cold and osmotic stress signaling determinants in Arabidopsis
1530-1600		Coffee Break
1600-1630		Jeffrey Skinner (Oregon State University, Corvallis, USA) Identification and characterization of a large CBF gene family in barley and its association with cold tolerance traits in the Triticeae
1630-1700		Julio Salinas (INIA, Madrid, Spain) The RCI5 gene from Arabidopsis encodes a flavin-contaning-monooxygenase that is involved in freezing and salt tolerance
1700-1730		André Laroche (Agriculture and Agri-Food Canada, Lethbridge, Canada) Low temperature gene regulation in winter wheat
1730-1900		Poster Session #1

Monday, July 12, 2004
0700-0845		Breakfast (Sapporo Aspen Hotel)
0900-1130		Poster Session #2
1130-1300		Lunch (provided)
1300-2000		Excursion to Otaru, an old port-town on the coast of the Japan Sea (dinner included)

Tuesday, July 13, 2004
0700-0845		Breakfast (Sapporo Aspen Hotel)
0845-1215		Session #2: Genomics & Metabolomics of Cold Response (Chair, Tapio Palva)
0845-0930		Seung Rhee (Carnegie Institution of Washington, Stanford, USA) Cold acclimation gene expression and promoter database
0930-1015		Gordon Gray (University of Saskatchewan, Saskatoon, Canada) Cold acclimation initiates a global reprogramming of the metabolome in Arabidopsis
1015-1045		Coffee Break
1045-1115		Eric Stockinger (The Ohio State University, Wooster, USA) Comparative genomic sequence, and expression analyses of CBF genes coincident with QTLs for cold hardiness from winter and spring barley cultivars differing in their freezing tolerance
1115-1145		Larry Gusta (University of Saskatchewan, Saskatoon, Canada) Transcript, proteomic and hormonal profiling of spring and winter Brassica napus leaves cold acclimated under natural and artificial conditions
1145-1215		Charles Guy (University of Florida, Gainesville, USA) Parallel transcriptome and metabolite profiling of the cold and heat shock response of Arabidopsis: seeing a bigger picture
1215-1400		Lunch
1400-1700		Session #3: Genetics & Genetic Applications of Cold Tolerance (Chair, Vaughan Hurry)
1400-1445		Patrick Hayes (Oregon State University, Corvallis, USA) Unraveling the barley winterhardiness regulon
1445-1530		Fathey Sarhan (University of Quebec, Montreal, Canada) Regulatory role of the MADS-box transcription factors during cold acclimation in cereals
1530-1600		Coffee Break
1600-1630		Yves Castonguay (Agriculture and Agri-Food Canada, Sainte-Foy, Canada) Identification of genetic polymorphisms associated with cold tolerance in alfalfa using candidate genes
1630-1700		Tony Chen (Oregon State University, Corvallis, USA) Enhanced freezing tolerance in transgenic potato plants by ectopic over-expression of AtCBF1 - morphological, anatomical, and physiological alterations
1700-1730		Yutaka Sato (National Agricultural Research Center for Hokkaido Region, Sapporo, Japan) Over-expression of a heat-inducible APX gene confers chilling tolerance to rice plants
1730-1900		Poster Session #3

Wednesday, July 14, 2004
0700-0845		Breakfast (Sapporo Aspen Hotel)
0845-1215		Session #4: Physiology & Biochemistry of Cold Acclimation (Chair, Rajeev Arora)
0845-0930		Eliot Herman (Donald Danforth Plant Science Center, St. Louis, USA) Second phase enhancement of cold acclimation involves complex biological mechanisms
0930-1015		Masaya Ishikawa (National Institute of Agrobiological Sciences, Tsukuba, Japan) Exploration for mechanisms involved in freezing behavior of cold hardy plant tissues
1015-1045		Coffee Break
1045-1115		Daisuke Takezawa (Hokkaido University, Sapporo, Japan) Physiological and morphological alterations associated with development of freezing tolerance in the moss Physcomitrella patens
1115-1145		Alina Kacperska (Warsaw University, Warsaw, Poland) Cell wall modifications in leaves of herbaceous plants in relation to plant acclimation to low temperatures
1145-1215		Annick Bertrand (Agriculture and Agri-Food Canada, Sainte-Foy, Canada) Impact of atmospheric CO2 enrichment on cold tolerance and overwintering reserves in black spruce and alfalfa
1215-1400		Lunch
1400-1700		Session #5: Cold Acclimation Mechanisms of Woody Plants (Chair, Tony Chen)
1400-1445		Tapio Palva (University of Helsinki, Helsinki, Finland) Control of growth and cold acclimation in silver birch
1445-1530		Vaughan Hurry (Umeå University, Umeå, Sweden) Cold acclimation and dormancy in poplar: insights from functional genomics
1530-1600		Coffee Break
1600-1630		Carl Fossdal (Norwegian Forest Research Institute, Ås, Norway) The maternal temperature during zygotic embryogenesis influences the adaptive properties of Norway spruce progenies; an epigenetic-like "memory" involving differential transcription of spruce genes?
1630-1700		Seizo Fujikawa (Hokkaido University, Sapporo, Japan) Functional role of winter-accumulated proteins in mulberry tree for adaptation to freezing
1800-2030		Conference Banquet (Sapporo Grand Hotel)
1930-2030		Banquet Lecture (Introduced by Patrick Hayes) Michael Thomashow (Michigan State University, East Lansing, USA) Regulatory networks involved in plant cold acclimation

Thursday, July 15, 2004
0700-0845		Breakfast (Sapporo Aspen Hotel)
0845-1145		Session #6: Membrane Aspects of Cold Acclimation & Freezing Injury (Chair, Ikuo Nishida)
0845-0930		Dirk Hincha (Max Planck Institute, Potsdam, Germany) Role of compatible solutes in plant freezing tolerance: in-vitro and in-vivo approaches to a complex problem
0930-1015		Karen Koster (The University of South Dakota, Vermillion, USA) Dehydration in model membranes and protoplasts: contrasting effects at low, intermediate and high hydrations
1015-1045		Coffee Break
1045-1115		Yoko Tominaga (Iwate University, Morioka, Japan) Effect of plasma membrane-associated proteins on acquisition of freezing tolerance in Arabidopsis thaliana
1115-1145		Ryozo Imai (National Agricultural Research Center for Hokkaido Region, Sapporo, Japan) Molecular characterization of phospholipid biosynthesis genes during cold acclimation in wheat
1145-1245		Seminar Review & General Discussion (Chairs, Tony Chen and Karen Tanino)
1245-1300		Closing Remarks (Seizo Fujikawa and Matsuo Uemura)
1300		Departure

List of Posters

[P-1]	Proteome analysis of abscisic acid-induced freezing tolerance in rice suspension cultured cells Aiko Morishita, Reiko Shinkawa, Kazuyuki Kuchitsu, Masaya Ishikawa

[P-2]	Cold acclimation in carrots (Daucus carota L. cv Nerac). Induction and changes in the cell wall during storage Federico Gómez Galindo, Luisa Elias, Maggie Smallwood, Marianne Sommarin, Ingegerd Sjöholm

[P-3]	Cold-hardening increases chilling resistance and Ca²⁺-ATPase activity in chilling-sensitive sweet corn seedlings Cunfu Lu, Ling-cheng, Jian, Ji-hong Li, Paul H. Li

[P-4]	Effects of cold calcium signaling and abscisic acid treatment on antifreeze protein synthesis and freezing tolerance in carrot (Daucus carota) suspension cultures Xi Chen, Cunfu Lu, Meiqin Liu, Yuzhen Chen, Xujia Zhang

[P-5]	Exogenous application of glycinebetaine increases the chilling tolerance capacity of tomato plants through enhanced catalase activity Eung-Jun Park, Zoran Jeknić, Tony H.H. Chen

[P-6]	Expression of wheat catalase in the transgenic rice plants reduces chilling-induced damage to membranes Haruo Saruyama, Hidenori Onodera, Matsuo Uemura

[P-7]	Fine mapping of qLTG-3-1, a major quantitative trait locus controlling low temperature germinability, in rice (Oryza sativa L.) K. Fujino, H. Sekiguchi, M. Yano

[P-8]	Genetic engineering of glycinebetaine synthetic capacity into tomato results in protection of plants, flowers, and seeds from chilling injury Eung-Jun Park, Zoran Jeknić, Atsushi Sakamoto, Jeanine DeNoma, Raweewan Yuwansiri, Norio Murata, Tony H.H. Chen

[P-9]	Mechanisms of temperature acclimation for photosynthesis in spinach leaves Wataru Yamori, Yukari Asakura, Masato Nakai, Ko Noguchi, Ichiro Terashima

[P-10]	Dynamics of the thermal oscillation in the spadix of skunk cabbage, Symplocarpus foetidus Takanori Ito, Kikukatsu Ito

[P-11]	Cold acclimation in the moss Physcomitrella patens Anzu Minami, Manabu Nagao, Keiichi Ikegami, Tomokazu Koshiba, Keita Arakawa, Seizo Fujikawa, Daisuke Takezawa

[P-12]	Recovery from freezing: histological and biophysical evidence for differential survival of tissues within oat crowns D.P. Livingston, S.P. Tallury, R. Premkumar, S. Owens, C.R. Olien

[P-13]	The binding of copper ions to plant glycine-rich proteins (GRPs) Masakatsu Kamiya, Yasuhiro Kumaki, Katsutoshi Nitta, Norio Matsushima

[P-14]	Ultrastructural and extracellular protein changes in cell suspension cultures of Populus euphratica associated with low temperature-induced cold acclimation Cunfu Lu, Meiqin Liu, Yuzhen Chen, Huanqin Dai, Hui Zhang, Xujia Zhang

[P-15]	Isolation and characterization of genes encoding fructosyltransferase in perennial ryegrass (Lolium perenne L.) Hiroshi Hisano, Akira Kanazawa, Sohei Kobayashi, Midori Yoshida, Keisuke Kitamura, Toshihiko Yamada

[P-16]	Comparison of Arabidopsis gene expression profiles between recovery from low-temperature stress and rehydration from dehydration using microarray Youko Oono, Motoaki Seki, Masakazu Satou, Junko Ishida, Kei Iida, Tetsuya Sakurai, Kenji Akiyama, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

[P-17	QTL analysis for winter hardiness in perennial ryegrass (Lolium perenne L.) and comparative genetics with cereals Toshihiko Yamada, Hiroshi Shinozuka, Hiroshi Hisano, Elizabeth S. Jones, John W. Forster

[P-18]	Functional analysis of monomeric isocitrate dehydrogenases from psychrophilic and mesophilic bacteria Seiya Watanabe, Yoshiaki Yasutake, Isao Tanaka, Yasuhiro Takada

[P-19]	The aminoalcoholphosphotranseferase (TaAAPT1) for phospholipids biosynthesis from crown of wheat: gene regulation, intracellular localization and enzymatic functions during cold acclimation Keita Sutoh, Takeshi Sakaki, Ryozo Imai

[P-20]	RNA helicase, CrhR of Synechocystis sp. PCC 6803 regulates the cold-inducible expression of heat shock genes Jogadhenu S. S. Prakash, Yu Kanesaki, Iwane Suzuki, Norio Murata

[P-21]	Understanding the mechanism of chilling-induced cytoplasmic acidification in a chilling-sensitive plant. 1. Introduction of mathematical model to the H⁺-accumulation into vacuole Yukio Kawamura, Heven Sze

[P-22]	Understanding the mechanism of chilling-induced cytoplasmic acidification in a chilling-sensitive plant. 2. Chilling-induced breakdown of PPi-dependent DpHvac-stat system directly results in the cytoplasmic acidification Yukio Kawamura, Heven Sze

[P-23]	Effects of low temperature and abscisic acid on the expression of sorbitol-6-phosphate dehydrogenase in Rosaceae fruit trees Yoshinori Kanayama, Misuzu Watanabe, Deguchi Michihito, Shohei Yamaki, Koki Kanahama

[P-24]	Low temperature-dependent transposition of Tam3 is associated with subcellular localization of the transposase in Antirrhinum majus Shin-nosuke Hashida, Tetsuo Mikami, Yuji Kishima

[P-25]	Rice homologs of the barley low temperature-responsive gene blt101 are only induced by drought stress and differentially expressed in response to treatment with ABA, methyl jasmonate, and ethephon Takashi Akiyama

[P-26]	Approaches to chilling tolerance in maize Jeff Ahrens, Paul Loida, Mark Abad, John Xu

[P-27]	Temporary increase in freezing tolerance of Arabidopsis T87 suspension cultured cells during cold acclimation Yutaka Sasaki, Riichiro Yoshida, Kazuo Shinozaki, Matsuo Uemura

[P-28]	Changes in freezing tolerance, enzyme activity and soluble carbohydrate content during cold acclimation in orchardgrass (Dactylis glomerata L.) Yasuharu Sanada, Tomoyuki Takai, Toshihiko Yamada

[P-29]	The starch-related alpha-glucan/water dikinase gene is involved in cold-inducible accumulation of soluble sugars and development of freezing tolerance during cold acclimation in Arabidopsis thaliana Ryoichi Yano, Masanobu Nakamura, Ikuo Nishida

[P-30]	Isolation and characterization of the freezing tolerant 1 mutant of Arabidopsis thaliana Ryoichi Yano, Masanobu Nakamura, Ikuo Nishida

[P-31]	Identification of proteins enriched in the overwinter buds of the gentian plant: their possible involvement in cold tolerance Ken-ichi Tsutsumi, Miho Takahashi, Takashi Hikage, Tetsuro Yamashita, Yasushi Saitoh, Motonobu Endou

[P-32]	Temperature fluctuation in Fraxinus pennsylvanica var. Subintegerrima and its surrounding environment A.M. Shirazi, S. H. Vogel

[P-33]	Cryoprotection in plant tissues related to reduced ice expansion Takashi Suzuki, Daisuke Kami, Katsuji Oosawa, Locksley E. McGann

[P-34]	Two dimensional electrophoretic analysis of dehydrin in rice seeds and seedlings under low temperature Michiko Momma

[P-35]	Characterization of low temperature-induced genes and promoters from wheats A. Laroche, M. Frick, D. Nilsson, C. Penniket, R. Quaghebeur, S. Garige, B. Puchalski, T. Despins, S. Huang, B. Genswein, F. Eudes, R. Graf, L. Robert, T. Ouellet, J. Singh, D.A. Gaudet

[P-36]	Theobroxide protects seedlings against chilling and drought Joshua D. Klein, Yonit Hebbe, Teruhiko Yoshihara

[P-37]	Comparison of intracellular soluble sugars between xylem cells that exhibit deep supercooling and cortical cells that exhibit extracellular freezing in birch Jun Kasuga, Keita Arakawa, Seizo Fujikawa

[P-38]	A type of cryopreservation makes the growth of regenerated plantlets vigorous Daisuke Kami, Sumiko Eda, Takashi Suzuki, Katsuji Oosawa

[P-39]	Subcellular localization of the compatible solutes in wheat seedlings during the first- and second-phase of cold hardening: comparisons among cultivars with different freezing tolerance Takashi Kamata, Matsuo Uemura

[P-40]	Functional characterization of cold shock domain proteins in plants Ryozo Imai, Dale Karlson, Kentaro Nakaminami

[P-41]	Dissection of the biochemistry, physiology and genetics of cold acclimation in pea V. Fontaine, S. Alvarez, S. Fiévet, H. Sellier, M. Thomas, R. Devaux, B. Delbreil, G. Grenier de March, C. Sarazin, C. Vuylsteker, G. Etévé, I. Lejeune-Hénaut

[P-42]	Ultrastructural observation of plant shoot apices under liquid nitrogen in the vitrification protocol using Cryo-SEM and TEM combined with non-aqueous freeze-substitution method Daisuke Tanaka, Seizo Fujikawa, Takao Niino, Matsuo Uemura

[P-43]	Phenolic changes during induction of cold hardiness in Ammopiptanthus mongolicus seedlings Meiqin Liu, Yiyin Chen, Cunfu Lu

[P-44]	Cloning of a novel dehydrin gene associated with cold acclimation in Ammopiptanthus mongolicus using solid subtractive hybridization Meiqin Liu, Xin Shen, Cunfu Lu, Weilun Yin

[P-45]	Application of differential thermal analysis to study supercooling in plants A.M. Shirazi, M. V. Thierry

[P-46]	Characterization of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor Kyonoshin Maruyama, Mie Kasuga, Masaru Ohme-Takagi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki

[P-47]	Abscisic acid induced increase of oligosaccharides and freezing tolerance in moss Physcomitrella patens Manabu Nagao, Kazuyuki Oku, Minoru Sakurai, Anzu Minami, Young-Min Kim, Atsuo Kimura, Takashi Suzuki, Kaoru Mizuno, Keita Arakawa, Seizo Fujikawa, Daisuke Takezawa

[P-48]	Winter hardiness of wheat cultivar 'Münstertaler' in Hokkaido, Japan Norio Iriki, Akira Kawakami, Zenta Nishio, Midori Yoshida

[P-49]	Induction of extracellular proteins in xylem tissue in birch during seasonal cold acclimation Keita Arakawa, Hiroshi Takashima, Jun Kasuga, Seizo Fujikawa

[P-50]	Overexpression of the wheat fructosyltransferase genes increases amounts of fructan and freezing tolerance of Lolium perenne plants Hiroshi Hisano, Akira Kanazawa, Akira Kawakami, Midori Yoshida, Yoshiya Shimamoto, Toshihiko Yamada

[P-51]	Identification and expression analyses of genes expressed in xylem ray parenchyma cells of Larix kaempferi that respond to cold environment by deep supercooling Naoki Takata, Keita Arakawa, Daisuke Takezawa, Seizo Fujikawa

[P-52]	Cloning of cDNAs of wheat fructosyltransferases associated with fructan accumulation during hardening and sugar induction of gene expression of sucrose:sucrose 1-fructosyltransferase Midori Yoshida, Dingbo Lin, Akira Kawakami

[P-53]	Possible role of an interaction between LIP19 and OsOBF1 during cold stress in rice Hidekazu Shimizu, Kazuhito Sato, Thomas Berberich, Atsushi Miyazaki, Ryozo Imai, Tomonobu Kusano

[P-54]	Trehalose biosyntheis in rice and its implication in chilling response Shuhei Shima, Md. H. R. Pramanik, Satoshi Tahara, Ryozo Imai

[P-55]	The effect of temperature and photoperiod on photosynthesis and the development of dormancy in contrasting ecotypes of dogwood (Cornus sericea L.) Gordon R. Gray, Kirk M. Cherry, Jillian N. Baerr, Jeremy D. Thomas, Robert K. Stevenson, Gift Marufu, Manjula S. Bandara, Karen K. Tanino

[P-56]	Expression and biochemical characterization of Arabidopsis cold shock domain proteins Myung Hee Kim, Dale Karlson, Ryozo Imai

[P-57]	Expression of early light-inducible proteins during cold hardening in wheat Etsuo Shimosaka, Hirokazu Handa

[P-58]	Comparative analysis of ESTs from cold acclimated and non-acclimated leaves of Rhododendron Hui Wei, Anik L. Dhanaraj, Lisa J. Rowland, Yan Fu , Stephen L. Krebs, Rajeev Arora

[P-59]	Macroarray approach for identification of novel genes involved in cold acclimation in Triticum aestivum Nikolai Christov, Petya Christova, Ryozo Imai

[P-60]	Effect of plasma membrane-associated proteins on acquisition of freezing tolerance in Arabidopsis thaliana Yoko Tominaga, Chihaya Nakagawara, Matsuo Uemura

[P-61]	Parallel transcriptome and metabolite profiling of the cold and heat shock response of Arabidopsis: seeing a bigger picture Charles Guy, Fatma Kaplan, Joachim Kopka, Dong Yul Sung, Dale Haskell, Nicole Gatzke, Wei Zhao

Posters submitted by June 16, 2004, are listed.